The ENGICOIN proposal aims at the development, from TRL3 to TRL5, of three new microbial factories (MFs), integrated in an organic waste anaerobic digestion (AD) platform, based on engineered strains exploiting CO2 sources and renewable solar radiation or H2 for the production of value-added chemicals, namely:
MF.1) the cyanobacteria Synechocystis to produce lactic acid from either biogas combustion flue gases or pure and costless CO2 streams from biogas-to-biomethane purification.
MF.2) the aerobic and toxic metal tolerant Ralstonia eutropha to produce PHA bioplastics from biogas combustion flue gases and complementary carbon sources derived from the AD digestate.
MF.3) the anaerobic Acetobacterium woodii to produce acetone from the CO2 stream from biogas-to-biomethane purification.