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Photoautotrophic growth of Synechocystis sp. PCC 6803 in a flat-panel photobioreactor, run in turbidostat mode under increasing intensities of orange-red light (636 nm), showed a maximal growth rate (0.12 h−1) at 300 μmolphotons m−2 s−1, whereas first signs of photoinhibition were detected above 800 μmolphotons m−2 s−1. To investigate the dynamic modulation of the thylakoid proteome in response to photoinhibitory light intensities, quantitative proteomics analyses by SWATH mass spectrometry were performed by comparing thylakoid membranes extracted from Synechocystis grown under low-intensity illumination (i.e. 50 μmolphotons m−2 s−1) with samples isolated from cells subjected to photoinhibitory light regimes (800, 950 and 1460 μmolphotons m−2 s−1). We identified and quantified 126 proteins with altered abundance in all three photoinhibitory illumination regimes.
These data reveal ...
Synechocystis gathered momentum in modelling studies and biotechnological applications owing to multiple factors like fast growth, ability to fix carbon dioxide into valuable products, and the relative ease of genetic manipulation. Synechocystis physiology and metabolism, and consequently, the productivity of Synechocystis-based photobioreactors (PBRs), are heavily light modulated. Here, we set up a turbidostat-controlled lab-scale cultivation system in order to study the influence of varying orange–red light intensities on Synechocystis growth characteristics and photosynthetic activity. Synechocystis growth and photosynthetic activity were found to raise as supplied light intensity increased up to 500 μmol photons m−2 s−1 and to enter the photoinhibition state only at 800 μmol photons m−2 s−1. Interestingly, reverting the light to a non-photo-inhibiting intensity unveiled Synechocystis to be able to promptly recover. Furthermore...
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We have identified and characterized the AccS multidomain sensor kinase that mediates the activation of the AccR master regulator involved in carbon catabolite repression (CCR) of the anaerobic catabolism of aromatic compounds in Azoarcus sp. CIB. A truncated AccS protein that contains only the soluble C-terminal autokinase module (AccS′) accounts for the succinate-dependent CCR control. In vitro assays with purified AccS′ revealed its autophosphorylation, phosphotransfer from AccS′∼P to the Asp60 residue of AccR, and the phosphatase activity toward its phosphorylated response regulator, indicating that the equilibrium between the kinase and phosphatase activities of AccS′ may control the phosphorylation state of the AccR transcriptional regulator. Oxidized quinones...
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Consejo Superior de Investigaciones Cientificas
The Spanish National Research Council is the largest public institution dedicated to research in Spain and will participate to the ENGICOIN project via the Department of Environmental Biology of The Biological Research Centre, where living systems are studied on every scale by multi-disciplinary experimental approaches. The Biological Research Centre (CIB) is a public funded national research institute that belongs to the Spanish National Research Council (CSIC). At the CIB´s Department of Environmental Biology, we are devoted to advance the understanding on how living organisms, plants, animals and microorganisms, interact and respond to their environment. Organisms are studied on every scale, from molecular to whole-body level, and using multidisciplinary experimental approaches.
Team role and expertise in the project:
CSIC will mainly participate as co-leader (together with BIOP) in the metabolic R. eutropha engineering and bioreactor development for PHA production within the microbial factory MF 2. CSIC will have a minor role in modelling, data monitoring and control strategy definition as well as products, microorganisms, materials and plants durability assessment in the integrated phase. Finally, CSIC will be active on the exploitation of results as well as on dissemination, communication and training tasks.
Auxiliadora PrietoPrincipal Investigator
Kungliga Tekniska Hoegskolan
The Royal Institute of Technology (KTH), based in Stockholm, is Sweden’s premier technical university. KTH has previously collaborated with ENGICOIN partner UvA to develop a microfluidic- based screening platform for engineered cyanobacteria, which will be used in the ENGICOIN project to screen lactate-producing mutant strains. Participation in the ENGICOIN project is through the KTH School of Biotechnology, which is located at the Science for Life Laboratory in Stockholm.
Team role and expertise in the project:
KTH will be active in the development of a photosynthetic cell factory producing lactate. Paul Hudson’s group at KTH has expertise in metabolic engineering of cyanobacteria, including developing state-of–the-art gene editing tools, genome scale metabolic modelling, and systems-biology analysis of gene expression during stress. KTH will be the partner responsible for the training activities related to ENGICOIN project.
Paul HudsonPrincipal Investigator